Mechanosensation of Tight Junctions Depends on ZO-1 Phase Separation and Flow
نویسندگان
چکیده
منابع مشابه
Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctions.
Defects in myosin VIIa lead to developmental anomalies of the auditory and visual sensory cells. We sought proteins interacting with the myosin VIIa tail by using the yeast two-hybrid system. Here, we report on shroom2, a submembranous PDZ domain-containing protein that is associated with the tight junctions in multiple embryonic and adult epithelia. Shroom2 directly interacts with the C-termin...
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Zonula occludens-1 (ZO-1) was the first tight junction protein to be cloned, and has been implicated as an important scaffold protein. It contains multiple domains that bind a diverse set of junction proteins. However, the molecular functions of ZO-1 and related proteins such as ZO-2 and ZO-3 have remained unclear. We now show that gene silencing of ZO-1 causes a delay of ~3 hours in tight junc...
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Jessica L. Maiers, Xiao Peng*, Alan S. Fanning and Kris A. DeMali The Interdisciplinary Graduate Program in Molecular and Cellular Biology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, USA The Department of Biochemistry, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, USA The Department of Cell Biology and Ph...
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The formation of a barrier between epithelial cells is a fundamental determinant of cellular homeostasis, protecting underlying cells against pathogens, dehydration and damage. Assembly of the tight junction barrier is dependent upon neighboring epithelial cells binding to one another and forming adherens junctions, but the mechanism for how these processes are linked is poorly understood. Usin...
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Temporal phase discrimination was measured as a function of spatial separation of the stimulus components. In contrast to many previous studies, phase discrimination thresholds were measured directly, rather than inferred from the ability to discriminate synchronous from antiphase stimuli, or from segregation or shape tasks. For abutting bars, relative phase thresholds were closely proportional...
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ژورنال
عنوان ژورنال: Cell
سال: 2019
ISSN: 0092-8674
DOI: 10.1016/j.cell.2019.10.006